Standardization of clinical enzyme assays.

Standardization of clinical enzyme assays.

During 1969, discussions between the Standards Subcommittee of the Laboratory Equipment and Methods Advisory Group of the Department of Health and Social Security and representatives of professional societies concerned with the assay of enzymes for diagnostic purposes led to the establishment of a working party to consider the current state of accuracy and reliability of diagnostic enzymology a...

Standardization of clinical enzyme assays: a reference method for aspartate and alanine transaminases.

Standardization of serum fructosamine assays.

We have calibrated a secondary serum protein standard by use (as primary standards) of samples of albumin and polylysine glycated with [14C]glucose in vitro, the glycation of which was assessed by radioactivity measurements and by elementary analysis for C and N. Using this standard for calibration in our improved fructosamine assay, one obtains an average fructosamine value of 247 mumol/L for ...

Standardization of immunoglobulin M capture enzyme-linked immunosorbent assays for routine diagnosis of arboviral infections.

Immunoglobulin M antibody-capture enzyme-linked immunosorbent assay (MAC-ELISA) is a rapid and versatile diagnostic method that readily permits the combination of multiple assays. Test consolidation is especially important for arthropod-borne viruses (arboviruses) which belong to at least three virus families: the Togaviridae, Flaviviridae, and Bunyaviridae. Using prototype viruses from each of...

The need for standardization of tacrolimus assays.

BACKGROUND Owing to the lack of an internationally recognized tacrolimus reference material and reference method, current LC-MS and immunoassay test methods used to monitor tacrolimus concentrations in whole blood are not standardized. The aim of this study was to assess the need for tacrolimus assay standardization. METHODS We sent a blinded 40-member whole-blood tacrolimus proficiency panel...